Representativeness and robustness of TCR repertoire diversity assessment. Mus musculus

Given the extreme diversity of TCR repertoires, understanding how the sequencing conditions, including cell numbers, biological and technical sampling and sequencing depth, impact the experimental outcome is critical to properly exploit these powerful data. Here we assessed the representativeness and robustness of TCR repertoire diversity assessment according to experimental conditions.

鉴于T细胞受体（TCR）库具有极强的多样性，明确测序相关条件（包括细胞数量、生物学与技术采样流程以及测序深度）对实验结果的影响，对于合理开发利用这类高价值数据至关重要。本研究针对不同实验条件，评估了TCR库多样性评估的代表性与稳健性。