NIST's Integrated Colony Enumerator

This work involves only data processing software and does not involve data to publish. Data are acquired by the users of this software by the following methods. Two methods can be used to acquire images of agar plates with bacterial colonies grown. In the first, plates were placed on a flatbed scanner, covered by soft gloss paper (HP LaserJet soft gloss paper), and 8-bit grayscale images of the plates were obtained at 300 dpi (dots per inch; 118 dots per cm), 600 dpi (236 dots per cm), and 1,200 dpi (472 dots per cm). In the second approach, images are acquired with a digital camera. Images are acquired at the maximum resolution of the camera with no zoom. More details are described in the manual for the software.

本研究仅涉及数据处理软件，不涉及可供发布的实验数据。相关数据由本软件用户通过以下两种方式获取：针对培养有细菌菌落的琼脂平板，可采用两种方法采集其图像。第一种方法为将平板置于平板扫描仪上，使用HP LaserJet 软光泽纸覆盖，分别以300 dpi (dots per inch，即每厘米118点)、600 dpi (每厘米236点)及1200 dpi (每厘米472点)的分辨率采集平板的8位灰度图像；第二种方法为使用数码相机采集图像，需采用相机的最大分辨率且不使用变焦功能。更多细节请参阅该软件的操作手册。