Preparation of PHB membranes by electrospinning and characterization for tissue engineering applications

ABSTRACT This work aims the preparing of mats composed of poly (3-hydroxybutyrate) -(PHB) biodegradable polymeric fibers, using the electrospinning technique. Mats were obtained under different conditions: changing the flow and temperature of the solution, tension and the addition of a surfactant to the solution. The obtained material was analyzed by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), thermogravimetry (TG), X ray diffractometry (XRD) and cell viability. The characterization by SEM showed that the best results, presenting fibers with constant diameters of 0.77 μm and without beads formation, were reached under the following conditions: concentration of 10% (m / v) solution in CF / DMF (9/1), solution flow of 5.8 ml/h, 17 kV and addition of surfactant (sodium dodecylsulfate, SDS). The results showed that the variation of the flow, tension and surfactant did not influence the average diameter of the fibers, but the increase of the temperature decreases this parameter. Moreover, the addition of a surfactant increases the density of the blankets and interconnect the pores, allowing cellular proliferation, and, thus. it is indicated for use as scaffolds.

摘要
本研究旨在采用静电纺丝技术制备由聚(3-羟基丁酸酯(poly(3-hydroxybutyrate), PHB)可生物降解高分子纤维构成的纤维毡。本研究通过调控溶液流速、溶液温度、纺丝电压以及向溶液中添加表面活性剂等工艺参数，制备得到不同条件下的纤维毡。对所制备的材料采用扫描电子显微镜(Scanning Electron Microscopy, SEM)、差示扫描量热法(Differential Scanning Calorimetry, DSC)、热重分析法(Thermogravimetry, TG)、X射线衍射法(X-ray Diffractometry, XRD)及细胞活力实验进行了表征。扫描电子显微镜表征结果显示，当采用以下工艺参数时可获得最优效果：所得纤维直径均一，直径为0.77 μm且无串珠结构，具体参数为：以9:1体积比的氯仿(CF)/二甲基甲酰胺(DMF)溶剂体系配制10%(m/v)浓度的溶液，溶液流速为5.8 ml/h，纺丝电压为17 kV，并添加表面活性剂十二烷基硫酸钠(Sodium Dodecylsulfate, SDS)。研究结果表明，溶液流速、纺丝电压以及表面活性剂的添加与否对纤维平均直径无显著影响，但升高纺丝温度会降低纤维平均直径。此外，添加表面活性剂可提升纤维毡的密度并连通其孔隙，有利于细胞增殖，因此该材料适合用作组织工程支架。