SW480 Signaling Hub Inhibition

In tumor cells, stepwise oncogenic deregulation of signaling cascades induces alterations of cellular morphology and promotes the acquisition of malignant traits. Using a panel of small molecular inhibitors, we established associated gene expression signatures of key signaling hubs. SW480 cells were grown in DMEM, 10% FCS, and treated for 24h with the following inhibitors or with solvent (DMSO): SU5402 (42uM); U0126 (10uM), SP600125 (20uM), LY294002 (20uM)

在肿瘤细胞中，信号级联反应的逐步致癌性失调会诱导细胞形态改变，并促进恶性特征的获得。本研究使用一组小分子抑制剂（small molecular inhibitors），构建了关键信号枢纽的关联基因表达特征谱。将SW480细胞培养于达尔伯克改良伊格尔培养基（DMEM）与10%胎牛血清（FCS）中，随后分别用下述抑制剂或溶剂对照二甲基亚砜（DMSO）处理24小时：SU5402（42μM）、U0126（10μM）、SP600125（20μM）、LY294002（20μM）。