Effects of exercise and Phellodendron amurense extract in prostate cancer. Effects of exercise and Phellodendron amurense extract in prostate cancer

Oxidative stress, induced by chronic prostatic inflammation, impairs normal protective mechanisms in the prostate and increases the risk for cancer progression. The Phellodendron amurense extract (Nexrutine®) had similar effects as exercise in reducing highly aggressive tumors in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. In this study, we examined changes in the transcriptome to understand how these two interventions affect prostate cancer. Overall design: Twenty-four, 8-10 weeks old male TRAMP mice, were randomized to control, exercise, and Phellodendron amurense extract intervention. Mice were maintained in a climate-controlled environment with a 12-hour light-dark cycle with diet and water provided ad libitum. Phellodendron amurense extract was supplied in the diet and running wheel was used as the exercise intervention. The control group mice were group housed had access to AIN-93 diet ad libitum and did not have access to running wheel. Body weight changes, food consumption and distance run on the running wheel were measured weekly for the 12-week intervention. At the end of the study, prostate tumors were excised, weighed, and processed for transcriptome analysis (3 replicates each condition).

慢性前列腺炎诱导的氧化应激可损伤前列腺的正常保护机制，升高癌症进展风险。黄柏提取物（Phellodendron amurense extract, Nexrutine®）在小鼠前列腺转基因腺癌（transgenic adenocarcinoma of the mouse prostate, TRAMP）模型中，展现出与运动相似的抑瘤效果，可有效降低高侵袭性肿瘤的负荷。本研究通过检测转录组表达变化，解析这两种干预方式影响前列腺癌进程的潜在分子机制。实验设计：将24只8~10周龄的雄性TRAMP小鼠随机分为对照组、运动组与黄柏提取物干预组。所有小鼠饲养于温控环境中，采用12小时光暗循环节律，自由采食与饮水。黄柏提取物通过日粮添加方式给药，运动干预采用跑轮训练。对照组小鼠群居饲养，仅自由采食AIN-93标准饲料，无跑轮使用权。在为期12周的干预周期内，每周监测小鼠体重变化、食物消耗量及跑轮跑动距离。实验结束时，摘除前列腺肿瘤并称量其重量，随后进行转录组分析（每组设置3个生物学重复）。