Expression data from BCL11A-null and wild-type bone marrow erythroid pregenitors

BCL11A is a critical mediator of hemoglobin switching and gamma-globin silencing. In this study, we showed the BCL11A is required in vivo for developmental silencing of gamma-globin genes in adult animals. We used microarray to determine the changes in gene expression profile after loss of BCL11A in adult erythroid cells CD71+Ter119+ erythroid progenitor cells were FACS-sorted from bone marrows of 6-week old control (Bcl11a +/+) and BCL11A knockout (Bcl11a fl/fl EpoR-Cre+) mice.

BCL11A是介导血红蛋白转换与γ-珠蛋白沉默的关键调控因子。本研究证实，BCL11A在成年动物体内是γ-珠蛋白基因发育性沉默所必需的。我们通过基因芯片（microarray）技术分析成年红系细胞中BCL11A缺失后的基因表达谱变化：实验所用的CD71+Ter119+红系祖细胞，通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）从6周龄对照（Bcl11a +/+）及BCL11A敲除（Bcl11a fl/fl EpoR-Cre+）小鼠的骨髓中分离获得。