Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples

We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.

本研究开发并验证了针对黏孢子虫寄生虫*Tetracapsuloides bryosalmonae*（该寄生虫为鱼类增生性肾病的致病菌）DNA的常规聚合酶链式反应（Polymerase Chain Reaction，PCR）与实时荧光定量聚合酶链式反应检测体系。本检测方法已在鱼类组织与野外采集的水样中开展验证实验，以评估其诊断检测性能与环境DNA检测能力。本检测方法的特异性、灵敏度及广谱适用性均优于此前用于鱼类组织及水样中T. bryosalmonae DNA检测的相关方法。