Sequencing clinical isolates from dental plaque. Streptococcus sobrinus TCI-157

Plaque samples were collected from twin children with and without dental caries. S. mutans and S. sobrinus were isolated by plating plaque scrapings on selective media. Unique isolates were chosen for genome sequencing by restriction digest genotyping; libraries were constructed using the EpiBio Nextera DNA sample prep kit, and sequencing was performed using an Illumina Genome Analyzer IIx.

本研究从患有龋齿与未患龋齿的双胞胎儿童中采集牙菌斑样本。将菌斑刮取物涂布接种于选择性培养基，分离得到变形链球菌（S. mutans）与远缘链球菌（S. sobrinus）。通过限制性酶切基因分型筛选独特的分离株用于基因组测序；使用EpiBio Nextera DNA样本制备试剂盒构建测序文库，并采用Illumina基因组分析仪IIx完成测序。