RNA-seq of pabpn1l ko and wt female mice oocytes and zygotes

After the library was qualified, the library was pooled according to the effective concentration and the demand of target offline data, and was sequenced by Illumina platform. Since PABPN1L can bind the poly(A) tail, we propose that it participates in the process of post-transcriptional regulation and degradation of maternal mRNA. We investigated the mRNA changes in GV oocytes, MII oocytes, and fertilized eggs using RNA-seq. GV oocytes, MII oocytes and zygotes were collected and mixed from three mice of each genotypes separately.

文库质检合格后，依据有效浓度与目标离线数据的需求完成文库混合，并通过Illumina测序平台进行测序。鉴于PABPN1L可结合poly(A)尾，我们推测其参与母源mRNA的转录后调控与降解过程。我们采用RNA-seq技术检测了GV卵母细胞、MII卵母细胞及受精卵内的mRNA表达变化。分别从每种基因型的3只小鼠体内采集上述三类细胞，并将同基因型的样本混合。