This is raw sequence data of Mycobacterium tuberculosis Methylation profile generated using PacBio Sequencing system.

Although Mycobacterium tuberculosis (Mtb) strains exhibit nucleotide level similarity of >99%, similarity is rarely replicated into the phenotype. Phenotypic heterogeneity in Mtb has been mostly associated to epigenetic inheritance. The most common epigenetic mechanism in Mtb is DNA methylation. At present the methylome of Mtb has not been completely characterized, neither has any resulting information been applied in inferring differences in clinical phenotypes observed in TB patients. Understanding the complete biology of Mtb will help in developing strategies for improving the Mtb treatment duration of the standard 6 months. This is the characterization of methylomes of 18 Mycobacterium tuberculosis (Mtb) isolates from patients in Blantyre, Malawi. This work presents the largest number of Mtb genomes to be completed in a single study using Single Molecule Real Time (SMRT) sequencing to date.

尽管结核分枝杆菌（Mycobacterium tuberculosis, Mtb）菌株的核苷酸水平相似度超过99%，但这种序列相似性极少能在表型层面得以体现。结核分枝杆菌的表型异质性多与表观遗传相关。结核分枝杆菌中最常见的表观遗传机制为DNA甲基化。目前，结核分枝杆菌的甲基化组尚未得到完整解析，相关研究所得信息也未被用于推断结核病患者临床表型的差异。解析结核分枝杆菌的完整生物学特性，将有助于开发优化标准6个月抗结核治疗疗程的相关策略。本研究对来自马拉维布兰太尔地区患者的18株结核分枝杆菌分离株的甲基化组进行了表征。截至目前，本研究是采用单分子实时（Single Molecule Real Time, SMRT）测序技术完成结核分枝杆菌基因组数量最多的单项研究。