Fecal microbiota transplantation treatment maintains post antibiotics gut function by change the gut microbiome and fecal metabolome to reduce gut inflammation and oxidative stress in nile tilapia (Oreochromis niloticus)

The imbalance of intestinal flora can affect the immune function and structural integrity of the intestinal barrier, leading to the colonization and reproduction of opportunistic pathogenic bacteria in the intestine to become the dominant flora, eventually inducing enteritis. This study aimed to investigate whether fecal microbiota transplantation (FMT) could improve the gut barrier in Nile tilapia (Oreochromis niloticus). The experiment involved administering normal saline (NS group) and fecal microbiota (FMT group) (from the negative control group (C group)) to tilapia that had been treated with oxytetracycline hydrochloride (OTC) (M group) by gavage. A total of 300 male tilapia (mean body weight 596.65 ± 47.18 g) were used, with 180 of them being fed OTC (120 mg/kg body weight/day) for 7 days to induce intestinal oxidative stress, while the rest served as the control group. After confirmation of mild chronic enteritis, the tilapia were treated in different ways.

肠道菌群失衡可影响肠道免疫功能与肠屏障结构完整性，致使肠道内机会致病菌定植繁殖并成为优势菌群，最终诱发肠炎。本研究旨在探讨粪便菌群移植（Fecal Microbiota Transplantation, FMT）能否改善尼罗罗非鱼（Oreochromis niloticus）的肠道屏障功能。实验以经盐酸土霉素（Oxytetracycline Hydrochloride, OTC）处理构建肠道氧化应激模型的尼罗罗非鱼（M组）为对象，分别对其灌胃生理盐水（NS组）与取自阴性对照组（C组）的粪便菌群制剂（FMT组）。本实验共纳入300尾雄性尼罗罗非鱼，平均体重为596.65±47.18 g，其中180尾以120 mg/kg体重/日的剂量投喂盐酸土霉素，连续7天以诱导肠道氧化应激，剩余120尾作为阴性对照组（C组）。待确认M组尼罗罗非鱼罹患轻度慢性肠炎后，再对各组开展差异化处理。