Transcript profiles in longissimus dorsi muscle and subcutaneous adipose tissue: A comparison of pigs with different post-weaning growth rates.. Sus scrofa domesticus

The objective of this experiment was to use transcriptional profiling of skeletal muscle and adipose tissue to develop a better understanding of the metabolic basis for poor weaned-pig transition. A total of 1,054 pigs were reared in commercial conditions and weighed at birth, weaning, and 3 weeks post- weaning. Transition average daily gain (tADG) was calculated as the average daily gain for the 3-week period post-weaning. Nine pigs from each of the lowest 10th percentile (low tADG) and the 60th-70th percentile (high tADG) were harvested at 3 weeks post-weaning. Differential expression analysis was conduced in both tissues using RNA-Seq methodology Overall design: mRNA profiling in two different tissues (skeletal muscle and adipose tissue) harvested at 3 weeks post-weaning

本实验旨在通过对骨骼肌（skeletal muscle）与脂肪组织（adipose tissue）进行转录组分析（transcriptional profiling），以深入解析仔猪断奶过渡期不良的代谢机制。
本研究共饲养1054头商品猪，分别于出生、断奶及断奶后3周时测定其体重。
过渡期平均日增重（transition average daily gain, tADG）以断奶后3周阶段的平均日增重计算所得。
从tADG最低的10%组与60%~70%组中各选取9头仔猪，于断奶后3周时屠宰采样。
采用RNA-Seq技术对两种组织开展差异表达分析（differential expression analysis）。
整体实验设计：于断奶后3周采集骨骼肌与脂肪组织，进行mRNA转录组分析（mRNA profiling）。