Cryo-electron microscopy EPN/Grenoble BAG

At the EPN campus level structural biology work is carried out in all the 4 institutes (IBS, ESRF, ILL and EMBL). Today all structural biology groups are turning
to cryo-EM. The EM demand is therefore extremely high and present in the 4 Institutes. However there are also some other Research Institutes in Grenoble and we are now proposing that the BAG is extended to them (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB)
In a nutshell, there are around 40 PIs that could benefit from Krios time on the EPN campus (more than 100 different samples). 2 Glacios are available on the EPN campus for pre-screening. Whereas some real structural work could be carried out on Glacios microscopes, for difficult samples the use of a krios is indispensable (heterogeneous sample or limited concentration requiring a large number of images; small protein etc). Only these samples will be selected for Krios time at the BAG level.

在EPN园区层面，结构生物学研究工作在全部4所研究所（IBS、ESRF、ILL及EMBL）中开展。如今，所有结构生物学团队均转向冷冻电镜技术（cryo-EM）。因此，这4所研究所对电镜（EM）的需求极为旺盛且普遍存在。然而，格勒诺布尔市还存在其他一些研究机构，我们现提议将BAG扩展至这些机构（细胞与植物生理学实验室LPCV、格勒诺布尔神经科学研究所GIN、高级生物科学研究所IAB）。

简言之，EPN园区内约有40位首席研究员（PI）可从Krios机时中受益（涉及超100种不同样本）。EPN园区内现有2台Glacios电镜用于预筛选。尽管Glacios电镜可开展部分实际结构研究工作，但对于复杂样本（异质样本、浓度受限需大量图像的样本、小蛋白等）而言，Krios电镜的使用不可或缺。仅此类样本将在BAG层面获得Krios机时。