METHODS FOR DISTINGUISHING AND IDENTIFYING PLANT VARIETIES

Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise， viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant.

本发明公开了两种方法：其一为通过在预设孵育时间与温度条件下，基于多糖水解酶（polysaccharide-hydrolyzing enzyme）活性测定多糖的部分水解程度，以区分和鉴定植物；其二为通过相同的检测手段，鉴定植物中异源多糖水解酶的来源生物体。该反应体系具备独特的化学与物理特性，可用于构建用于测定多糖水解酶活性的黏度曲线。通过比对不同植物的黏度曲线，即可区分或鉴定不同植物；同理，比对不同来源生物体的黏度曲线，也可鉴定植物中异源多糖水解酶的来源生物体。