Metabolic inhibition of Clostridium thermocellum M1570 by pentose sugars.

Differential RNA-Seq analyses to investigate the basis for metabolic inhibition of Clostridium thermocellum M1570 by xylose. The M1570 strain was developed in the C. thermocellum DSM 1313 ?hpt background strain. Lactate dehydrogenase (Ldh) and phosphotransacetylase (Pta) genes are deleted (Argyros DA, Tripathi SA, Barrett TF, Rogers SR, Feinberg LF, Olson DG, Foden JM, Miller BB, Lynd LR, Hogsett DA, Caiazza NC: High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes. Appl Environ Microbiol 2010, 77:8288-8294 ) Overall design: Differential RNA-Seq analyses was used to investigate the basis for metabolic inhibition of Clostridium thermocellum M1570 by xylose. Cultures were grown in serum bottles containing 40 mL of MTC medium with or without 15 g/L xylose and were harvested at mid-exponential phase. Quadruplicate cultures were used for each experimental condition tested.

本数据集旨在通过差异RNA测序（Differential RNA-Seq）分析，探究木糖对热纤梭菌（Clostridium thermocellum）M1570的代谢抑制机制。M1570菌株是以热纤梭菌DSM 1313 Δhpt为背景菌株构建所得，其乳酸脱氢酶（Lactate dehydrogenase, Ldh）与磷酸转乙酰酶（phosphotransacetylase, Pta）基因均已被敲除（Argyros DA, Tripathi SA, Barrett TF, Rogers SR, Feinberg LF, Olson DG, Foden JM, Miller BB, Lynd LR, Hogsett DA, Caiazza NC: High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes. Appl Environ Microbiol 2010, 77:8288-8294）。总体实验设计：实验菌株接种于含40 mL MTC培养基的血清瓶中，培养基分别添加或不添加15 g/L木糖，于指数生长中期收集菌体；每个实验条件均设置四个生物学重复。