Pyruvate kinase M1 and M2 isoform knock in ES cells differentiate into three germ layers. Mus musculus

Analysis of wild type, PKm1 KI, and PKm2 KI ES cells. Overall design: Each ES cells were differentiated into three germ layer using embryoid body formation culture. Total RNAs were isolated from embryoid body at day 0, 3, and 7

野生型、PKm1基因敲入（PKm1 KI）及PKm2基因敲入（PKm2 KI）胚胎干细胞（Embryonic Stem Cells, ES cells）的分析。实验整体设计：通过拟胚体（Embryoid Body, EB）形成培养体系，将各胚胎干细胞诱导分化为三胚层细胞；分别于第0、3、7天从拟胚体中提取总RNA。