Transcriptomic study of cold adaptation mechanisms in Chlamydomonas nivalis

We employ a unicellular snow alga Chlamydomonas nivalis to explore its cold adaptation mechanisms during temperature fluctuation, and apply a reference species of the same genus, C. reinhardtii as a comparative object. C. nivalis UTEX 2824 and C. reinhardtii CC-124 cells were grown at 22 C till the mid-log phase and transferred to 4 C for 1 h. Cultures were collected before (0h) and after (1h) the cold treatment and used for RNA extraction.

本研究采用单细胞雪生藻类雪衣藻（Chlamydomonas nivalis），探究其在温度波动过程中的冷适应机制，并选取同属的参考物种莱茵衣藻（Chlamydomonas reinhardtii，缩写C. reinhardtii）作为对照材料。将雪衣藻UTEX 2824与莱茵衣藻CC-124的细胞于22摄氏度条件下培养至对数生长中期，随后转移至4摄氏度条件下处理1小时。分别于冷处理前（0小时）与冷处理后（1小时）收集培养液，用于RNA提取。