ADAD2 facilitates piRNA biogenesis and spermiogenesis in mice. ADAD2 facilitates piRNA biogenesis and spermiogenesis in mice

Adenosine Deaminase Domain Containing 2 (ADAD2) is a testis-specific protein composed of a double-stranded RNA binding domain and a non-catalytic adenosine deaminase domain. A recent study showed that ADAD2 is indispensable for the male reproduction in mice. However, its detailed function remains elusive. The aims of this study are to investigate the cause of male sterility in Adad2 knockout mice and to understand the molecular function of ADAD2. RNA-seq analyses were carried out to analyze the changes in the transcriptome and piRNA population of Adad2 mutant testes. Overall design: RNA-seq of testis from Adad2-knockout mouse

含腺苷脱氨酶结构域2（ADAD2）是一种睾丸特异性蛋白，由双链RNA结合结构域与非催化型腺苷脱氨酶结构域组成。近期研究表明，ADAD2对小鼠的雄性生殖功能不可或缺，但其具体分子功能仍未明确。本研究旨在探究Adad2基因敲除小鼠雄性不育的成因，并解析ADAD2的分子功能。本研究通过RNA测序（RNA-seq）分析了Adad2突变型睾丸的转录组与piRNA（Piwi互作RNA）群体的变化。整体实验设计：对Adad2基因敲除小鼠的睾丸开展RNA测序。