Transcript Profiling of Common Bean Embryo Regions at Globular Stage Using Laser Capture Microdissection Coupled with RNA-Seq

We used laser capture microdissection (LCM) to capture globular stage common bean embryo proper and suspensor, and profiled the transcriptome of these two embryo regions using next-generation sequencing. Our long-term goal is to understand the region-specific differentiation processes that occur during early embryo development and how genes are activated specifically in the suspensor and embryo proper. Overall design: Illumina sequencing of transcripts from laser-captured embryo proper and suspensor regions of common bean globular stage embryo. Two biological replicates were collected for each embryo region.

本研究采用激光捕获显微切割（laser capture microdissection, LCM）技术，分离得到菜豆球形期胚胎的胚体与胚柄，并通过下一代测序对这两个胚胎区域的转录组进行分析。本研究的长期目标为解析早期胚胎发育过程中的区域特异性分化进程，以及基因如何在胚柄与胚体中被特异性激活。整体实验设计：对激光捕获获得的菜豆球形期胚胎的胚体与胚柄区域的转录本开展Illumina测序；每个胚胎区域均设置两个生物学重复。