Clostridium tetani biomarkers for culture condition. Clostridium tetani biomarkers for culture condition

We used RNA-seq to determine Clostridium tetani gene expression changes in response to culture conditions and time. Changes in response to time were more pronounced than those in response to culture conditions. The tetanus toxin gene is always highly expressed but does show expression changes between culture conditions. These results may become part of an approach to reduce animal testing during vaccine manufacturing. Overall design: 39 samples, comprising 6 growth conditions and 5 time points, with some replicates.

本研究采用RNA测序（RNA-seq）技术，解析破伤风梭菌（Clostridium tetani）在不同培养条件及培养时间下的基因表达变化。相较于培养条件引发的基因表达差异，培养时间对基因表达的调控效果更为显著。破伤风毒素基因始终维持高表达水平，但在不同培养条件下仍存在表达量变化。该研究结果可作为疫苗生产环节减少动物实验的潜在解决方案之一。实验整体设计：共包含39个样本，涵盖6种生长条件与5个时间点，并设置了部分生物学重复。