Anti-pathogenic and probiotic-friendly effect of benzyloxy pyridine derivative impregnated into bacterial cellulose matrix

Bacterial cellulose (BC) has emerged as a promising biomaterial for biomedical applications due to its unique properties. In this study, firstly, the potential of BC as a carrier material for the originally synthesized benzyloxy pyridine derivative (BeOxP), and also an antimicrobial and probiotic bacteria-friendly (on <i>Lactiplantibacillus plantarum</i> 299v (LP299V^®) (DSM 9843)) effect of BC- BeOxP was investigated. The BC- BeOxP composite material was developed using a simple and efficient method, ensuring the uniform dispersion of BeOxP within the BC matrix. Characterization of BeOxP has been carried out with High-Resolution Mass Spectroscopy (HRMS) and Nuclear Magnetic Resonance (NMR). Additionally, BC- BeOxP was characterized by Thermogravimetric analysis (TGA), Differential thermal analysis (DTA), Scanning Electron Microscopy (SEM), Texture analysis, and Fourier-transform infrared spectroscopy (FTIR), confirming the successful integration of BeOxP without compromising the structural integrity of BC. The mechanical properties of the BC- BeOxP composites were also evaluated to ensure their suitability for drug-carrying or food-packaging applications. Antimicrobial activity of the BC- BeOxP was analyzed against <i>Staphylococcus aureus</i> ATCC 29213 and <i>Escherichia coli</i> ATCC 25922 while the probiotic-friendly effect was tested on <i>Lactiplantibacillus plantarum</i> 299v (LP299V^®) (DSM 9843). The findings open avenues for further research of BC-based drug carriers or packaging materials for advanced biomedical applications.

细菌纤维素（Bacterial cellulose, BC）凭借其独特的理化性能，已成为生物医学应用领域极具发展潜力的生物材料。本研究首先探究了BC作为合成得到的苄氧基吡啶衍生物（benzyloxy pyridine derivative, BeOxP）载体材料的潜力，以及BC-BeOxP复合材料的抗菌性能与对植物乳杆菌299v（LP299V®）（DSM 9843）的益生友好性。本研究采用简便高效的方法制备了BC-BeOxP复合材料，实现了BeOxP在BC基质中的均匀分散。采用高分辨质谱（High-Resolution Mass Spectroscopy, HRMS）与核磁共振（Nuclear Magnetic Resonance, NMR）对BeOxP进行了结构表征；此外，通过热重分析（Thermogravimetric analysis, TGA）、差热分析（Differential thermal analysis, DTA）、扫描电子显微镜（Scanning Electron Microscopy, SEM）、质地分析与傅里叶变换红外光谱（Fourier-transform infrared spectroscopy, FTIR）对BC-BeOxP复合材料进行了表征，证实BeOxP成功负载于BC基质中，且未破坏BC的结构完整性。同时评估了BC-BeOxP复合材料的力学性能，以验证其适用于载药或食品包装领域。针对金黄色葡萄球菌（Staphylococcus aureus）ATCC 29213与大肠埃希氏菌（Escherichia coli）ATCC 25922分析了BC-BeOxP复合材料的抗菌活性，并以植物乳杆菌299v（LP299V®）（DSM 9843）为模型验证了其益生友好效果。本研究结果为基于BC的载药载体或包装材料在先进生物医学领域的后续研究提供了新的方向。