Assessment of Microvessel Isolation Protocol by Western Blot to Verify Capillary Enrichment

Western blot images and quantification of samples generated from whole murine brain (yellow), vessels collected after enrichment (blue), and microvessels collected after enrichment and filtration (pink). Protein samples were probed for the relative levels of alpha-smooth muscle actin (aSMA), Claudin5, and platelet-endothelial cell adhesion molecule-1 (PECAM-1), with alpha-tubulin referenced as the loading control. This analysis provided greater insight into the output from each collection technique with respect to microvessel content.

本数据集包含小鼠全脑组织（黄色组）、富集后收集的血管（蓝色组）以及经富集与过滤步骤后收集的微血管（粉色组）的蛋白质印迹（Western blot）图像及样本定量结果。实验以α-微管蛋白（alpha-tubulin）作为上样内参，对各样本中α-平滑肌肌动蛋白（alpha-smooth muscle actin, aSMA）、紧密连接蛋白5（Claudin5）以及血小板-内皮细胞黏附分子-1（platelet-endothelial cell adhesion molecule-1, PECAM-1）的相对表达水平进行了检测。本分析可针对微血管组分特征，深入解析不同样本收集方法的产出效果差异。