Induction_of_mutation_rate_in_mouse_pedigrees_by_ionising_radiation. Induction_of_mutation_rate_in_mouse_pedigrees_by_ionising_radiation

Deep (>25X) whole genome sequencing of 20 mice from pedigrees in which a male mouse has been exposed to 3Gy of ionising radiation (acute X-rays) 4 days or 8 weeks before conceiving pups. The two timepoints are to assess the impact of IR on pre-meiotic (i.e. spermatognoial stem cells) and post-meiotic (i.e. sperm) gametes. At each timepoint, each irradiated male (C57BL/6J) was mated with two females (CBA/Ca). The 20 mice will be selected for sequencing on the basis of maximising the number of independent meioses studied at the two timepoints. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/本数据集包含20只小鼠的>25倍深度全基因组测序（whole genome sequencing）数据。这些小鼠来源于特定家系，其雄性亲本在受孕幼崽前4天或8周接受了3戈瑞的急性X射线电离辐射（ionising radiation）暴露。设置两个时间节点的核心目标为评估电离辐射对减数分裂前（pre-meiotic，即精原干细胞spermatogonial stem cells）及减数分裂后（post-meiotic，即精子）配子（gametes）的影响。各时间节点下，每只受辐照的C57BL/6J品系雄性小鼠均与两只CBA/Ca品系雌性小鼠交配。本次参与测序的20只小鼠将以最大化两个时间点下可研究的独立减数分裂事件数量为原则进行筛选。本数据属于预出版发布数据集。如需了解威康信托桑格研究所（Wellcome Trust Sanger Institute）共享的预出版数据的规范使用方法（含相关出版暂缓（publication moratoria）细则），请访问http://www.sanger.ac.uk/datasharing/