Spike times from intracellular responses to step currents in juvenile zebra finch caudal mesopallium

Data from intracellular recordings obtained from neurons in the caudal mesopallium of 52 juvenile (30-35 days post hatch) zebra finches of both sexes. All procedures were performed according to National Institutes of Health guidelines and protocols approved by the University of Virginia Institutional Animal Care and Use committee.Details of rearing conditions and data collection are given in the associated publication. The spike_times.zip archive contains files corresponding to one intracellular recording epoch from a single neuron. Filenames follow the format {cell_id}_{epoch}.pprox. Each epoch comprises 20 sweeps. Each sweep was 6 s in duration: 100 ms at 0 pA, a 2000-ms depolarizing step, 1000 ms at 0 pA, 500 ms at -20 pA, 500 ms at -40 pA, 500 ms at -20 pA, and 1400 ms at 0 pA. The amplitude of the depolarizing pulse was systematically varied within each epoch over a range that was set so that at least 10 sweeps in each epoch evoked action potentials without eliciting depolarization block. The range of current steps was adjusted throughout the recording as needed.The data are stored in a custom JSON-based format called pprox. Briefly, the JSON object contains metadata about the recording at the top level, and a field called "pprox" that comprises a list of objects corresponding to individual sweeps. Each sweep contains a field called "events" with a list of the times of the spikes that occurred during the trial, along with estimates of the membrane potential (Vm), series resistance (Rs), and input resistance (Rm).The Python code used to produce the pprox files from the raw intracellular recording data and to analyze the results is available on github.

本数据集涵盖52只雌雄两性幼年斑胸草雀（孵化后30~35天）尾侧中脑皮层（caudal mesopallium）神经元的细胞内记录数据。所有实验操作均严格遵循美国国立卫生研究院（National Institutes of Health）指南，并通过弗吉尼亚大学机构动物护理与使用委员会的伦理审批。饲养条件与数据采集的详细信息参见相关发表文献。spike_times.zip压缩包内的每份文件对应单个神经元的一次细胞内记录时段（epoch），文件名遵循{cell_id}_{epoch}.pprox格式。每个记录时段包含20次试次（sweep），单一试次总时长为6秒，时序配置如下：前100毫秒维持0皮安（pA）电流，随后施加2000毫秒的去极化电流阶跃，接着依次为1000毫秒0 pA、500毫秒-20 pA、500毫秒-40 pA、500毫秒-20 pA，最后1400毫秒维持0 pA电流。每个记录时段内，去极化脉冲的幅值会被系统地调整，调整范围的设置需确保该时段内至少有10次试次可诱发动作电位且不会引发去极化阻滞。电流阶跃的范围可根据记录过程中的实际需求进行动态调整。本数据集采用自定义的基于JSON格式的pprox文件格式存储。简而言之，顶层JSON对象包含本次记录的元数据，其中名为"pprox"的字段为存储各试次对应信息的对象列表。每个试次对象均包含名为"events"的字段，该字段存储了该试次期间记录到的动作电位时间序列，同时包含膜电位（Vm）、串联电阻（Rs）与输入电阻（Rm）的估算值。用于从原始细胞内记录数据生成pprox文件并开展结果分析的Python代码已开源至GitHub平台。