Sirtuin assay of purified Hos2 preparations using resveratrol: UPDATE 1
收藏Figshare2014-05-27 更新2026-04-29 收录
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https://figshare.com/articles/dataset/Sirtuin_assay_of_purified_Hos2_preparations_using_resveratrol_UPDATE_1/1031579
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资源简介：
Fluorimetric Sirtuin1 like deacetylase assay with rHos2 protein using Fluor de Lys®−Sirt1 substrate in presence of Sirtuin1 activator Reserveratrol. This experiment is carried out in presence of HDAC inhibitor Trichostatin to inhibit any residual Histone deacetylase activity. Updated from: http://dx.doi.org/10.6084/m9.figshare.841658.
本实验以重组Hos2蛋白（rHos2 protein）为实验样本，采用Fluor de Lys®−Sirt1底物，在Sirtuin1激活剂白藜芦醇（Reserveratrol）存在的条件下，开展类Sirtuin1脱乙酰酶的荧光测定实验。为抑制体系中残留的组蛋白脱乙酰酶活性，本实验同时添加了组蛋白脱乙酰酶（HDAC）抑制剂曲古抑菌素（Trichostatin）。本数据集更新自：http://dx.doi.org/10.6084/m9.figshare.841658.
创建时间：
2014-05-27
