Pond water SIP fraction microbial communities from Walnut Creek, CA, United States - JGIPond_F_60pct_30ng_2_1.7475 metagenome

SIP metagenome fraction of pond sample from Joint Genome Institute, Walnut Creek spiked with Pseudomonas putida & Escherichia coli bacterial isolates (for each isolate 30ng of DNA labeled at 60pct with C13 were added); replicate number 2. This fraction has a SIP fraction number 5 and DNA density of 1.7475 g/uL. This is part of a larger study to identify the limit of detection of JGI SIP metagenomics pipeline.

本数据集为来自联合基因组研究所（Joint Genome Institute，JGI）核桃溪池塘样本的稳定同位素探针（Stable Isotope Probing，SIP）宏基因组组分。实验中向该样本掺入了恶臭假单胞菌（Pseudomonas putida）与大肠杆菌（Escherichia coli）的细菌分离株：每份分离株添加30ng以60%比例被碳13标记的DNA，本样本为第2次重复。该SIP组分编号为5，其DNA密度为1.7475 g/μL。本数据集属于一项旨在探明JGI SIP宏基因组分析流程检出限的大型研究的一部分。