Plant DNA metabarcoding data of Lake Ulu

Here is the amplicon data of ancient plant DNA from a long sediment core of Lake Ulu (the Oymyakon region, eastern Siberia). PCR was performed with the “g” and “h” universal plant primers for the P6 loop region of the chloroplast trnL (UAA) intron (Taberlet et al., 2007). We produced three PCR replicates for sediment samples and DNA extraction controls and added a PCR negative template control (NTC) in each PCR replicate batch. Library preparation and next-generation sequencing (2×150 bp) on the Illumina NextSeq 500 platform were performed with two independent sequencing runs at Fasteris SA, Switzerland. The data from two runs were merged into a single dataset for the final interpretation.

本数据集为西伯利亚东部奥伊米亚康地区乌鲁湖长沉积岩芯中的古代植物DNA扩增子数据。实验采用针对叶绿体trnL(UAA)内含子P6环区域的"g"与"h"型通用植物引物进行PCR扩增（Taberlet等，2007）。我们为沉积样本及DNA提取对照设置了三次PCR重复，并在每一批次PCR重复实验中加入了PCR阴性模板对照（NTC）。文库构建及基于Illumina NextSeq 500平台的二代测序（2×150 bp）由瑞士Fasteris SA公司通过两次独立测序实验完成。将两次测序得到的数据合并为单一数据集，用于最终的数据分析与解读。