miRNA profiling of Adenosine treated Endothelial Progenitor Cells (EPCs)

Studies of miRNA profiling in early and late endothelial progenitor cells treated or not by cardioprotective nucleoside adenosine. Early outgrowth endothelial progenitor cells were obtained by adhesion of peripheral blood mononuclear cells of healthy volunteers. Late endothelial progenitor cells were obtained by purification of CD34+ peripheral blood cells and were cultured and amplified in endothelial-specific medium containing growth factors. Both cell types were treated by adenosine (10micromol/L) for 6 hours. Total RNA was extracted using mirVana Kit and quantified by Nanodrop. RNA was labeled and hybridized using Agilent miRNA Complete Labeling and Hyb Kit. 3 to 4 arrays per sample were hybridized and scanned with the Genepix 4000B Scanner (Molecular Devices). Six independent experiments were performed.

本数据集针对经心脏保护性核苷腺苷处理与未处理的早期增殖性内皮祖细胞及晚期内皮祖细胞开展microRNA（miRNA）表达谱研究。早期增殖性内皮祖细胞通过健康志愿者外周血单个核细胞贴壁培养法获取；晚期内皮祖细胞则通过纯化CD34+外周血细胞，并在添加生长因子的内皮专用培养基中培养扩增得到。两类细胞均以10μmol/L腺苷处理6小时。总RNA采用mirVana试剂盒提取，并通过Nanodrop进行定量分析。使用Agilent miRNA Complete Labeling and Hyb Kit完成RNA标记与杂交实验，每个样本设置3至4张阵列进行杂交，随后利用Genepix 4000B扫描仪（Molecular Devices公司）完成扫描。本研究共计开展6次独立重复实验。