Citrate addition enhanced anaerobic hydrocarbon degrader activity

Citrate addition can increase phosphorus and hydrocarbon bioavailability to enhance hydrocarbon bioremediation, but the overall microbial community response is poorly understood. In order to elucidate the potential mechanisms of citrate addition for hydrocarbon biostimulation, the structure and function of microbial communities were investigated in a gasoline-contaminated site in calcareous cold region soil.There are three treatments (no amendment, phosphate amendment, phosphate and citrate amendment) for the biostimulation deliveries. DNA was extracted from soils for different biostimulation treatments. 16S metagenomics sequencing library was constructed with primers 926F and 1392R and was sequenced on an Illumina Miseq sequencer.

添加柠檬酸盐可提升磷与烃类的生物可利用性，从而强化烃类生物修复，但目前学界对微生物群落的整体响应机制仍知之甚少。为阐明柠檬酸盐添加用于烃类生物刺激修复的潜在机制，本研究针对钙质寒区土壤中的一处汽油污染点位，探究了其微生物群落的结构与功能特征。本实验设置了三种生物刺激修复施加处理：无添加对照、磷添加处理，以及磷与柠檬酸盐联合添加处理。从不同生物刺激修复处理的土壤样本中提取基因组DNA。采用引物926F与1392R构建16S宏基因组测序文库，并使用Illumina Miseq测序仪完成测序。