DataSheet1_Salivary proteomic profile of young healthy subjects.PDF

Background: The incidence of noncommunicable diseases (NCDs) has been rapidly ramped up worldwide. Hence, there is an urgent need to non-invasively detect NCDs possibly by exploiting saliva as a ‘liquid biopsy’ to identify biomarkers of the health status. Since, the absence of standardized procedures of collection/analysis and the lack of normal ranges makes the use of saliva still tricky, our purpose was to outline a salivary proteomic profile which features healthy individuals.
Methods: We collected saliva samples from 19 young blood donors as reference population and the proteomic profile was investigated through mass-spectrometry.
Results: We identified 1,004 proteins of whose 243 proteins were shared by all subjects. By applying a data clustering approach, we found a set of six most representative proteins across all subjects including Coronin-1A, F-actin-capping protein subunit alpha, Immunoglobulin J chain, Prosaposin, 78 kDa glucose-regulated protein and Heat shock 70 kDa protein 1A and 1B.
Conclusion: All of these proteins are involved in immune system activation, cellular stress responses, proliferation, and invasion thus suggesting their use as biomarkers in patients with NCDs.

Background: 非传染性疾病（noncommunicable diseases, NCDs）的发病率在全球范围内快速攀升。因此，亟需通过无创手段检测非传染性疾病，其中可利用唾液作为“液体活检（liquid biopsy）”样本，以识别与健康状态相关的生物标志物。当前，由于缺乏标准化的采集与分析流程，且未建立正常参考范围，唾液的临床应用仍存在诸多挑战。本研究旨在明确健康个体的唾液蛋白质组学特征。
Methods: 本研究招募19名年轻献血者作为参考人群采集唾液样本，并通过质谱（mass-spectrometry）技术分析其蛋白质组表达谱。
Results: 本研究共鉴定出1004种蛋白质，其中243种为所有受试者所共有。通过数据聚类分析方法，我们筛选出6种在所有受试者中均具有代表性的核心蛋白质，分别为冠蛋白1A（Coronin-1A）、F-肌动蛋白加帽蛋白亚基α（F-actin-capping protein subunit alpha）、免疫球蛋白J链（Immunoglobulin J chain）、前鞘脂激活蛋白（Prosaposin）、78 kDa葡萄糖调节蛋白（78 kDa glucose-regulated protein）以及热休克70 kDa蛋白1A和1B（Heat shock 70 kDa protein 1A and 1B）。
Conclusion: 上述所有蛋白质均参与免疫系统激活、细胞应激反应、细胞增殖与侵袭过程，提示其可作为非传染性疾病患者的潜在生物标志物。