Homo sapiens isolate:HeLa Raw sequence reads

Targeted DMS-MaPseq was applied to capture the intronic regions of the MYC pre-mRNA. DMS was used to modify RNA to generate mutations in cDNA sequence generated through mutational readthrough. Using the MYC reference sequence, the accumulation of mutated bases were quantified and normalized using RNAframework package. Processed probing data were used to characterize structures predicted within the introns of MYC pre-mRNA.

本研究采用靶向硫酸二甲酯修饰引物延伸测序（Targeted DMS-MaPseq）技术，以捕获MYC前体mRNA（MYC pre-mRNA）的内含子区域。利用硫酸二甲酯（DMS）对RNA进行修饰，使反转录生成互补DNA（cDNA）序列时通过突变通读引入碱基突变。以MYC参考序列为参照，借助RNAframework软件包（RNAframework）对突变碱基的累积量进行定量与归一化处理。最终将经处理的RNA结构探测数据用于解析MYC前体mRNA内含子区域内预测的RNA二级结构。