IKAROS re-expression modulates transcriptional networks in IKZF1-mutant Ph+ B-ALL [RNA-Seq]

With the goal to investigate tumor suppressor mechanisms regulated by IKAROS (IKZF1) in PH chromosome–positive B-cell acute lymphoblastic leukemia (B-ALL) harboring IKZF1 mutations. This dataset includes bulk RNA-seq profiles from human MXP5 and PDX2 cell lines, which were engineered to express doxycycline-inducible wild-type IKAROS (IK1) or an empty vector control. RNA was collected 24 hours post-induction to capture early transcriptional responses to IK1 re-expression. These data are part of a larger multi-omics study integrating RNA-seq, ChIP-seq, CUT&RUN, ATAC-seq, and HiChIP to define IKAROS-regulated transcriptional and chromatin networks in IKZF1-deficient Ph? B-ALL. Overall design: The experimental design includes two IKZF1-deficient Ph? B-ALL cell lines (MXP5 and PDX2) expressing either doxycycline-inducible wild-type IKAROS (IK1) or empty control vectors. Cells were treated with 1 µg/mL doxycycline for 24 hours prior to RNA extraction. Each condition was performed in biological duplicate, resulting in eight total RNA-seq libraries. This design supports comparisons between IK1-induced and control samples within and across cell lines to identify IKAROS-regulated transcriptional programs. *************************************************************** The table below lists GEO accessions reused/reanalyzed for this study. ***************************************************************

本数据集旨在探究IKAROS（IKZF1）在携带IKZF1突变的费城染色体阳性（Ph+）B细胞急性淋巴细胞白血病（B-ALL）中调控的肿瘤抑制机制。本数据集包含源自人源MXP5与PDX2细胞系的批量RNA测序（bulk RNA-seq）谱图数据，上述细胞系经工程改造后可表达多西环素诱导型野生型IKAROS（IK1）或空载载体对照。诱导后24小时收集RNA样本，以捕获IK1重新表达所引发的早期转录应答。本数据集属于一项大型多组学研究的一部分，该研究整合了RNA测序、染色质免疫沉淀测序（ChIP-seq）、CUT&RUN、转座酶可及性测序（ATAC-seq）以及HiChIP技术，旨在明确IKZF1缺陷型Ph？B-ALL中IKAROS调控的转录与染色质调控网络。

实验设计：本实验包含两株IKZF1缺陷型Ph？B-ALL细胞系（MXP5与PDX2），分别表达多西环素诱导型野生型IKAROS（IK1）或空载对照载体。在RNA提取前，使用1 μg/mL多西环素处理细胞24小时。每个实验条件均设置生物学重复，最终共获得8个RNA测序文库。该实验设计可实现在细胞系内及细胞系间对比IK1诱导组与对照组样本，从而鉴定IKAROS调控的转录程序。

****************************************************************
下表列出了本研究复用/重新分析的基因表达综合数据库（Gene Expression Omnibus, GEO）登录号。
****************************************************************