CRISPRi screen for a-amylase yield improvements in Bacillus subtilis

Our study showed that optimizing ncRNA expression can increase or lower the yield of alpha-amylase enzyme production in Bacillus subtilis while revealing a range of potentially novel ncRNAs. Overall design: RNA-seq data for bacterial amylase production in two strains (codon optimized v. control) with two fed-batch replicates on two days

本研究证实，优化非编码RNA（non-coding RNA，ncRNA）的表达水平，可上调或下调枯草芽孢杆菌（Bacillus subtilis）中α-淀粉酶（alpha-amylase）的酶产量，同时鉴定出一批潜在的新型非编码RNA。
实验整体设计：针对密码子优化菌株与对照菌株两种菌株的细菌淀粉酶生产体系，在两天内各设置两个补料分批培养重复样本，对其进行RNA测序（RNA-seq）并获取对应数据。