Human middle ear effusion microbiome; Targeted loci. Homo sapiens

The pathogenesis of chronic otitis media remains largely unknown. Though this is not typically considered an infection, varying genera of bacteria have been previously described in middle ear effusions (MEEs) from patients with chronic otitis media. Microbe analysis of MEEs using 16s rRNA sequencing allows detection of the bacterial genera within these samples and comparison of population and abundance of genera between samples, ultimately providing additional insight into the complex immunological response resulting in this condition.

慢性中耳炎（chronic otitis media）的发病机制在很大程度上仍未明确。尽管该病通常不被视为感染性疾病，但既往已有研究在慢性中耳炎患者的中耳积液（middle ear effusions, MEEs）中检出了多种不同菌属的细菌。采用16S rRNA测序（16s rRNA sequencing）对MEEs进行微生物分析，可检出此类样本中的细菌菌属，并能比对不同样本间各菌属的种群组成与丰度，最终为解析引发该疾病的复杂免疫应答过程提供新的研究视角。