Zhu_ tables _ESM 7.xlsx from Analysis of the regulation networks in grapevine reveals response to waterlogging stress and candidate gene-marker selection for damage severity

Owing to the climate change impacts, waterlogging is one of the most hazardous abiotic stresses to crops, which also can result in a serious reduction in the quantity and quality of grape berry and wine production during the rainy season. Therefore, the exploration of the mechanism of grape response to waterlogging is necessary, for which the analysis of the regulation networks' response to waterlogging stress in grapevine leaves at the transcriptomic level was carried out. In this study, 12 634 genes were detected in both waterlogging stress and control grapevine plants, out of which 6837 genes were differentially expressed. A comparative analysis revealed that genes functioning in the antioxidant system, glycolysis and fermentation pathway, chlorophyll metabolism, amino acid metabolism and hormones were activated to reduce injury to grapes under the waterlogging stress. Meanwhile, genes encoding class-2 non-symbiotic haemoglobin were determined as important in waterlogging acclimation. Additionally, the expression variations of three marker genes were found to be informative and can be used to predict the viability of the grapevines subjected to waterlogging. This research not only probes the molecular mechanism underlying grapevine waterlogging tolerance but also puts forward an idea about the application of gene expression information to practical management.

受气候变化影响，渍涝胁迫是危害作物最严重的非生物胁迫之一，在雨季还会大幅降低葡萄浆果与葡萄酒的产量及品质。因此，解析葡萄对渍涝胁迫的响应机制具有重要意义，本研究针对转录组层面的葡萄叶片渍涝胁迫响应调控网络开展了分析。本研究在渍涝处理与对照葡萄植株中共检测到12634个基因，其中6837个基因呈现差异表达。比较转录组分析结果显示，抗氧化系统、糖酵解与发酵通路、叶绿素代谢、氨基酸代谢以及激素相关基因在渍涝胁迫下被激活，以减轻葡萄受到的损伤。同时，编码2类非共生血红蛋白的基因被证实对葡萄渍涝驯化具有关键作用。此外，3个标记基因的表达变化可有效反映植株状态，可用于预测渍涝处理葡萄的存活能力。本研究不仅解析了葡萄耐涝性的分子机制，同时为基因表达信息在实际生产管理中的应用提供了新思路。