Global gene expression analysis of human embryonic stem cells, adult fibroblasts , and CD34+ cord blood (CB) cells before, during, and afer their episomal induction of pluripotency. Homo sapiens

Global gene expression analysis of (a) human embryonic stem cells, (b) adult fibroblasts with and without nucleofection of SOKM, and ( c ) CD34+ cord blood cells at various time points during induction of pluripotency with SOKM, with or without co-culture with bone marrow stromal cells (BMSC). Overall design: Total RNA was harvested from adult fibroblasts, adult fibroblasts three days after nucleofection with 4F (SOKM), Day -3 unstimulated cord blood (CB) cells, Day 0 growth factor stimulated CB cells, Day +3 CB cells with or without nucleofection at Day zero and with or without Day zero to Day +3 co-culture with bone marrow stromal cells (BMSC), Day 23 CB cells nucleofected on day zero and co-cultured on BMSC from Day Zero to Day +3, and undifferentiated H9 human embryonic stem cells. All experimental conditions repeated in three independent trials, and each replicate at each condition analyzed on an individual microarray.

本数据集为全局基因表达分析数据集，涵盖以下三类样本：（a）人类胚胎干细胞；（b）转染或未转染SOKM的成人成纤维细胞；（c）在使用SOKM诱导多能性的不同时间点的CD34阳性脐带血细胞，且此类细胞可选择是否与骨髓基质细胞（bone marrow stromal cells, BMSC）进行共培养。实验设计概述：从下述样本中提取总RNA：成人成纤维细胞、经4种因子（SOKM）核转染3天后的成人成纤维细胞、第-3天未激活的脐带血（cord blood, CB）细胞、第0天经生长因子激活的脐带血细胞、第+3天的脐带血细胞（可在第0天进行或不进行核转染，且可在第0天至第+3天进行或不进行与骨髓基质细胞的共培养）、第0天接受核转染且于第0天至第+3天在骨髓基质细胞上完成共培养的第23天脐带血细胞，以及未分化的H9人类胚胎干细胞。所有实验条件均设置3次独立生物学重复，每个条件下的每一份重复样本均通过独立的基因微阵列芯片进行检测分析。