Cryptorchidism in the orl rat is associated with muscle patterning defects in the fetal gubernaculum and altered hormonal signaling. Rattus norvegicus

This study was designed to provide additional insight into testicular hormone production and responsiveness in the orl strain and complement ongoing efforts to characterize the genetic basis of cryptorchidism in this isolated rat colony. Overall design: Gubernacula were harvested from Long Evans and ORL rat embryos at gestational day 17 and 19. RNA was purified from litter-based organ pools. 26 RNA samples were processed and hybridized to GeneChips. Please note that a mix-up of the samples gubernaculum-GD19-a and -b appears to have occurred as data from these samples clustered with the opposite strain than expected in hierarchical clustering analysis. Thus the strain information was annotated as 'unknown'.

本研究旨在为解析ORL品系大鼠的睾丸激素生成与应答特性提供新的见解，并为阐明该孤立大鼠种群中隐睾症的遗传基础补充相关数据，以支撑当前正在推进的相关表征工作。

实验设计：于妊娠第17天及第19天，从Long Evans与ORL品系大鼠的胚胎中收取睾丸引带（gubernaculum），以同窝胚胎的睾丸引带混合样本提取总RNA，共制备26份RNA样品并经处理后与GeneChips进行杂交。

请注意，本研究中GD19组的a、b两份睾丸引带样本出现标记混淆，经层级聚类分析发现其聚类结果与预期品系不符，因此将该两份样本的品系信息标注为“未知”。