A switch from neuronal network development to maintenance during postnatal development of rat prefrontal cortex

To unravel the gene expression changes during postnatal prefrontal cortex development, RNA-seq was performed in the rat medial prefrontal cortex at five time points from early life to adulthood (postnatal day 8, 14, 21, 35 and 70) and differential expression of protein-coding genes, lincRNAs and alternative exons was analyzed. A switch from neuronal network development to maintenance during postnatal rat prefrontal cortex development was shown. Overall design: Comparison of gene expression between 5 different developmental time points (postnatal day 8, 14, 21, 35 and 70) in medial prefrontal cortex of Wistar rats. 5 samples per time point.

为阐明出生后前额叶皮层发育过程中的基因表达变化，本研究对大鼠内侧前额叶皮层在从幼年到成年的5个时间点（出生后第8、14、21、35和70天）开展了RNA测序（RNA-seq），并对蛋白编码基因、基因间长链非编码RNA（lincRNAs）以及可变外显子的差异表达情况进行了分析。研究揭示了大鼠出生后前额叶皮层发育过程中，存在从神经元网络发育向网络维持的转换。实验设计概述：比较Wistar大鼠内侧前额叶皮层在5个不同发育时间点（出生后第8、14、21、35和70天）的基因表达水平，每个时间点设置5个生物学重复样本。