Vegetation variables and rhizosphere bacterial communities from ten paired sites in southwestern Finland, 2021

Plant invasions can have major impacts on ecosystems not only in aboveground but also in belowground through changes in microbiota. Particularly legumes, which often host nitrogen-fixing symbionts (rhizobia), can be expected to modify soil bacterial communities. We examined the effect of the invasive herbaceous legume, Lupinus polyphyllus, on the alpha diversity and community composition of soil bacteria. We also explored whether vegetation cover, the cover of other (non-invasive) legumes or number of vascular plants was associated with soil bacterial communities. We sampled rhizosphere soil and surveyed vegetation from ten paired sites (invaded more than ten years ago and uninvaded locations) in southwestern Finland, and identified bacterial DNA by using 16S rRNA gene amplicon sequencing. The plant invader and the three vegetation variables considered had no effect on soil bacterial alpha diversity in terms of bacterial richness, Shannon and Inverse Simpson diversity indices. The compo..., To examine the impact of the herbaceous legume Lupinus polyphyllus on soil bacterial communities, vegetation variables and rhizosphere samples were collected from ten paired sites (ten invaded and ten uninvaded locations) around the Turku area in southwestern Finland in June 2021. The uninvaded locations were at least 30 m apart from the invaded locations, and the distances between the ten sample sites varied from 1.7 to 32.7 km. Each location contained four sampling points of 1 × 1 m.
Bacterial DNA was identified by using 16S rRNA gene amplicon sequencing, resulting in 9897 amplicon sequence variants (ASVs) for analysis. To characterise soil bacterial diversity, bacterial richness (i.e., number of bacterial ASVs per sample), Shannon and inverse Simpson diversity indices were calculated based on the bacterial ASVs., , This Community_data2021_README file was generated on 2024-01-09 by Satu Ramula ()

1. Title of Dataset: Vegetation variables and rhizosphere bacterial communities from ten paired sites in southwestern Finland, 2021

2. Date of data collection: June 2021 (range: 2021-06-07 - 2021-06-10)

3. Geographic location of data collection: Turku area, Finland (latitude: 60.357-60.521, longitude: 22.168-22.740)

4. Information about funding sources that supported the collection of the data: Research Council of Finland (#331046)

5. Description of methods used for collection/generation of data:
To examine the impact of the invasive herbaceous legume Lupinus polyphyllus on soil bacterial communities, vegetation variables and rhizosphere samples were collected from ten paired sites (ten invaded and ten uninvaded locations) around the Turku area in southwestern Finland in June 2021. The uninvaded locations were at least 30 m apart from the invaded locations, and the distances between the ten s...

植物入侵可通过改变微生物群落，对生态系统产生地上与地下双重重大影响。其中，常与固氮共生体根瘤菌（rhizobia）形成共生关系的豆科植物，尤其有望重塑土壤细菌群落。本研究针对入侵性草本豆科植物多叶羽扇豆（Lupinus polyphyllus）对土壤细菌α多样性及群落组成的影响展开调查，同时探讨植被盖度、其他（非入侵性）豆科植物盖度以及维管束植物数量是否与土壤细菌群落存在关联。我们于芬兰西南部的10个配对样地（入侵历史超过10年的样地与未入侵样地）采集根际土壤并开展植被调查，并通过16S rRNA基因扩增子测序技术鉴定细菌DNA。研究发现，就细菌丰富度、香农（Shannon）指数与逆辛普森（Inverse Simpson）多样性指数而言，该入侵植物与所考量的3种植被变量均未对土壤细菌α多样性产生显著影响。群落组……

为探究草本豆科植物多叶羽扇豆对土壤细菌群落的影响，研究团队于2021年6月在芬兰西南部图尔库地区的10个配对样地（10个入侵样地与10个未入侵样地）采集植被变量与根际样本。未入侵样地与入侵样地间距至少为30米，10个采样点之间的距离介于1.7至32.7千米之间。每个样地包含4个1×1米的采样点。

通过16S rRNA基因扩增子测序技术鉴定细菌DNA，最终获得9897个扩增子序列变异（ASVs，amplicon sequence variants）用于后续分析。为表征土壤细菌多样性，基于细菌ASVs计算了细菌丰富度（即每个样本中的细菌ASVs数量）、香农指数与逆辛普森多样性指数。

本Community_data2021_README文件于2024年1月9日由Satu Ramula生成。

1. 数据集标题：芬兰西南部图尔库地区10个配对样地的植被变量与根际细菌群落（2021年）
2. 数据采集日期：2021年6月（时间范围：2021-06-07至2021-06-10）
3. 数据采集地理位置：芬兰图尔库地区（纬度：60.357-60.521，经度：22.168-22.740）
4. 数据采集资助方信息：芬兰研究理事会（项目编号：#331046）
5. 数据采集/生成方法说明：为探究入侵性草本豆科植物多叶羽扇豆对土壤细菌群落的影响，研究团队于2021年6月在芬兰西南部图尔库周边的10个配对样地（10个入侵样地与10个未入侵样地）采集植被变量与根际样本。未入侵样地与入侵样地间距至少为30米，10个采样点之间的距……