RNA-seq (SPIA-seq): Intestinal stem cell aging signature reveals a reprogramming strategy to enhance regenerative potential

The impact of aging on intestinal stem cells (ISCs) has not been fully elucidated. In this study, we identified widespread epigenetic and transcriptional alterations in old ISCs. Using a reprogramming algorithm, we identified a set of key transcription factors (Egr1, Irf1, FosB) that drives molecular and functional differences between old and young states. Overall, by dissecting the molecular signature of aged ISCs, our study identified transcription factors that enhance the regenerative capacity of ISCs. Full length transcriptional profiles of intestinal stem cells of young (2 month) and old (22 month) mice.衰老对肠道干细胞（intestinal stem cells，ISCs）的影响尚未完全阐明。本研究在老年肠道干细胞中鉴定出广泛存在的表观遗传与转录改变。通过重编程算法，我们筛选出一组关键转录因子（Egr1、Irf1、FosB），其可驱动年轻与衰老状态间的分子及功能差异。总体而言，本研究通过解析衰老肠道干细胞的分子特征，鉴定出可提升肠道干细胞再生能力的转录因子。本数据集包含年轻（2月龄）与老年（22月龄）小鼠肠道干细胞的全长转录组谱。