Comparison of D. simulans/D. sechellia F1 male hybrid genome-wide expression values to pure-species parents

Species often produce sterile hybrids early in their evolutionary divergence, and some evidence suggests that hybrid sterility may be associated with deviations or disruptions in gene expression. In support of this idea, many studies have shown that a high proportion of male-biased genes are underexpressed compared to non-sex-biased genes in sterile F1 male hybrids of Drosophila species. In this study, we examined and compared patterns of misexpression in 4 day old sterile male F1 hybrids of Drosophila simulans and its sibling species, D. sechellia. We analyzed hybrids using genome-wide D. melanogaster arrays from the Drosophila Genome Resource Center (DGRC). The results from a custom array (GPL4022) for this species pair, from the custom array and the genome-wide array for the D. simulans-D. mauritiana species pair, and from the larvae of these two species pairs using the custom array, are presented separately. Keywords: Comparison of pure-species Drosophila expression to hybrid expression We analyzed 11 arrays: 4 of sim-sec vs. sec, 4 of sim-sec vs. sim, and one each of self-hybridizations (sim vs. sim, sim-sec vs. sim-sec, sec vs. sec).

物种在演化分化早期常产生不育杂交后代，已有证据表明杂交不育可能与基因表达异常或紊乱相关。
为验证这一假说，多项研究显示，在果蝇（Drosophila）物种的不育F1雄性杂交后代中，相较于非性别偏向基因，高比例的雄性偏向基因呈现表达量下调现象。
本研究针对拟果蝇（Drosophila simulans）与其近缘物种塞氏果蝇（Drosophila sechellia）的4日龄不育雄性F1杂交后代，检测并比较了其基因异常表达（misexpression）模式。
我们采用果蝇基因组资源中心（Drosophila Genome Resource Center, DGRC）提供的全基因组黑腹果蝇（Drosophila melanogaster）芯片对杂交样本进行了分析。
本研究分别展示了针对该物种对的定制芯片（GPL4022）、拟果蝇-毛里求斯果蝇（D. simulans-D. mauritiana）物种对的定制芯片与全基因组芯片，以及采用定制芯片分析的上述两个物种对的幼虫样本所得的实验结果。
关键词：纯种果蝇基因表达与杂交后代基因表达的比较分析
本次分析共使用11张芯片：4张为拟果蝇-塞氏果蝇杂交样本与塞氏果蝇的比较（sim-sec vs. sec）、4张为拟果蝇-塞氏果蝇杂交样本与拟果蝇的比较（sim-sec vs. sim），以及3张自身杂交对照样本（分别为拟果蝇自身杂交、拟果蝇-塞氏果蝇杂交样本自身杂交、塞氏果蝇自身杂交）各1张。