Gain of chromatin loops marks the exit from naive pluripotency - ChIP seq for CTCF and Rad21. Gain of chromatin loops marks the exit from naive pluripotency - ChIP seq for CTCF and Rad21

ChIP-seq to map the binding sites for CTCF and cohesin subunit Rad21 in the naive mES cells (46C cell line grown in the 2i/LIF condition) and in the neural stem cells (derived from the 46C ES cells using the mono-layer differentiation protocol, grown in the N2B27 medium these cells are Nestin+). The naive mES cells were grown in two different media (fetal bovine serum, FBS and 2i/LIF culture - naive pluripotency conditions) as detailed in the growth protocols.

本数据集通过染色质免疫共沉淀测序（ChIP-seq）技术，定位CCCTC结合因子（CTCF）与黏连蛋白亚基Rad21在两类细胞中的全基因组结合位点：一类为采用46C细胞系、于2i/LIF培养条件下培养的幼稚型小鼠胚胎干细胞（naive mES cells）；另一类为通过单层分化方案由46C胚胎干细胞诱导获得、并在N2B27培养基中培养的神经干细胞（该细胞呈巢蛋白（Nestin）阳性）。前述幼稚型小鼠胚胎干细胞分别采用胎牛血清（FBS）与2i/LIF幼稚多能性两种培养基进行培养，具体培养细节详见对应的生长实验方案。