Expression data from distal colon tissues of WT and Gm31629-/- mice. Expression data from distal colon tissues of WT and Gm31629-/- mice

Mucosal healing is a key treatment goal for inflammatory bowel disease, and epithelial regeneration is required for an intact gut epithelium. Long noncoding RNAs are involved in the development of inflammatory bowel disease. Here, we investigated the role of a novel long noncoding RNA, Gm31629, in epithelial regeneration. We used RNA-seq to to detail the global programme of gene expression in distal colon tissues from WT and Gm31629-/-. Overall design: WT and Gm31629-/- mice were subjected to 3% DSS treatment in drinking water for 7 days and sacrificed on day 8. Distal colon tissues were dissected and total RNA was extracted for eukaryotic RNA-seq and associated analysis.

黏膜愈合（Mucosal healing）是炎症性肠病（inflammatory bowel disease）的核心治疗目标，而完整肠道上皮（gut epithelium）的维持依赖于上皮再生（epithelial regeneration）。长链非编码RNA（Long noncoding RNAs）参与炎症性肠病的发生发展。本研究聚焦一种新型长链非编码RNA Gm31629，探讨其在上皮再生过程中的调控作用。我们通过RNA测序（RNA-seq）详细阐明了野生型（WT）与Gm31629基因敲除（Gm31629-/-）小鼠远端结肠组织的全局基因表达程序。整体实验设计：野生型与Gm31629基因敲除小鼠均通过饮用水给予3%葡聚糖硫酸钠（DSS）造模，处理7天后于第8天处死。摘取远端结肠组织，提取总RNA用于真核RNA测序及后续关联分析。