Analysis of Mason-Pfizer Monkey Virus Gag Particles by Scanning Transmission Electron Microscopy

Mason-Pfizer monkey virus immature capsids selected from the cytoplasm of baculovirus-infected cells were imaged by scanning transmission electron microscopy. The masses of individual selected Gag particles were measured, and the average mass corresponded to 1,900 to 2,100 Gag polyproteins per particle. A large variation in Gag particle mass was observed within each population measured.

从感染杆状病毒的细胞细胞质中分离得到的梅森-普菲策尔猴病毒（Mason-Pfizer monkey virus）未成熟衣壳，通过扫描透射电子显微镜（scanning transmission electron microscopy）完成成像。研究人员对筛选出的单个Gag颗粒的质量进行了测定，结果表明每个颗粒的平均质量对应1900至2100个Gag多聚蛋白（Gag polyprotein）。在本次测量的各研究种群中，均观测到Gag颗粒的质量存在显著差异。