hTERT effects in CD8 T Lymphocytes HD1

Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD2 was profiled on U133Plus 2.0 and submitted as a separate GEO series. Keywords: Cell Line Comparison. triplicates of 4 conditions, 1 chip (hTERT_YOUNG_HD1_REP2) excluded because of low quality hybridization.

本研究利用过表达端粒酶（telomerase）的CD8+T淋巴细胞克隆，探究调控T细胞增殖的分子机制。实验对从两名年龄介于30至35岁的健康个体（HD1与HD2）体内分离得到的CD8+初始T细胞克隆（CD8+ naive T-cell clones）开展转导与亚克隆操作，将上述T细胞克隆转导以表达hTERT/GFP或单独表达绿色荧光蛋白（GFP）。针对HD2样本的基因表达谱分析采用U133Plus 2.0芯片完成，相关数据已作为独立的基因表达汇编（GEO, Gene Expression Omnibus）系列提交。关键词：细胞系比较（Cell Line Comparison）。本实验包含4种实验条件的生物学重复各3份，其中1份芯片样本（hTERT_YOUNG_HD1_REP2）因杂交质量过低被剔除。