Single cell RNA-sequencing reveals placenta cellular heterogeneity in adverse pregnancy

The placenta is a highly heterogeneous organ and is closely related to adverse pregnancy. The previous bulk sequencing of whole tissue could not show the characteristics of individual cells and the interactions between cells. Here, we select the placental tissues of the gestational diabetes group(GDM), preeclampsia group(PE), advanced age group(GL) and normal control group for single-cell sequencing in order to explain the mechanism of related diseases in more depth.nated spatial and temporal regulation of gene expression in the murine hindlimb determines the identity of mesenchymal progenitors and the development of diversity of musculoskeletal tissues they form. Hindlimb development has historically been studied with lineage tracing of individual genes selected a priori, or at the bulk tissue level, which does not allow for the determination of single cell transcriptional programs yielding mature cell types and tissues. To identify the cellular trajectories of lineage specification during limb bud development, we used single cell mRNA sequencing (scRNA-seq) to profile the developing murine hindlimb. Overall design: 2 placenta samples from PE, GDM, the elderly group and the control group respectively.

胎盘是一种高度异质性的器官，与不良妊娠结局密切相关。既往针对全组织的批量测序技术，无法揭示单个细胞的特征与细胞间的相互作用。本研究选取妊娠糖尿病组（GDM）、子痫前期组（PE）、高龄组（GL）及正常对照组的胎盘组织开展单细胞测序（single-cell sequencing），以期更深入阐释相关疾病的发病机制。
主导性的时空基因表达调控在小鼠后肢中决定了间充质祖细胞的身份，以及其形成的肌肉骨骼组织的多样性发育。长期以来，对后肢发育的研究多采用预先选定的单个基因的谱系示踪技术，或是在整体组织层面开展，此类方法无法确定产生成熟细胞类型与组织的单细胞转录程序。为鉴定肢芽发育过程中谱系特化的细胞轨迹，本研究采用单细胞mRNA测序（scRNA-seq）对发育中的小鼠后肢进行转录组谱型分析。
实验整体设计：分别从子痫前期组、妊娠糖尿病组、高龄组及正常对照组中各选取2份胎盘样本。