freshwater sediment metagenome Raw sequence reads

The field survey was conducted at Xinxue River mCW in February 2017. In this field survey, surface sediments and overlying water samples were collected from 6 sites (C0, C1, C2, C3, C4, C5) at retention pond export (117°9'8''E,34°45'59''N), primary wetland export (117°9'8''E,34°45'59''N), secondary wetland export (117°8'56''E,34°46'7''N), tertiary wetland export (117°8'44''E,34°45'40''N), quartus wetland export (117°8'23''E,34°45'1''N) and fifth wetland export (117°9'9''E,34°44'17''N). At each site, the overlying water samples were gathered with Niskin bottles and filtered through 0.45µm syringe filters. The surface sediment samples were collected with a Peterson mud sampler by taking 0-10 cm depth and put in a sterile polyethylene plastic bag under 4 o C. The samples were transported to the laboratory for the follow-up experiments. Then, sediment samples were freeze-dried and sieved for further analysis.

本野外调查于2017年2月在新雪河mCW展开。本次调查共设置6个采样点位（C0、C1、C2、C3、C4、C5），分别位于滞留池出口（东经117°9'8''、北纬34°45'59''）、一级人工湿地出口（东经117°9'8''、北纬34°45'59''）、二级人工湿地出口（东经117°8'56''、北纬34°46'7''）、三级人工湿地出口（东经117°8'44''、北纬34°45'40''）、四级人工湿地出口（东经117°8'23''、北纬34°45'1''）及五级人工湿地出口（东经117°9'9''、北纬34°44'17''）。每个点位均采用尼斯金采水器采集上覆水样品，经0.45μm针式过滤器过滤。表层沉积物样品通过彼得森采泥器采集0~10 cm深度的沉积物，装入无菌聚乙烯塑料袋并置于4℃环境保存。所有样品均被运送至实验室开展后续实验，随后沉积物样品经冷冻干燥、筛分后用于进一步分析。