Exploration of the Mechanism by Which Bacterial Volatiles Alleviate Salt Stress in Cucumber

Taking the "bacterial volatiles - cucumber - salt stress" interaction system as the research object and relying on transcriptomics and metabolomics technologies, this study sets the core objectives as follows: By comparing the data of cucumber leaves and roots among the salt stress group, bacterial volatiles + salt stress group, and control group, screen the differentially expressed genes and characteristic metabolites regulated by bacterial volatiles; establish a "gene - metabolite" regulatory network with the help of multi - omics correlation analysis, clarify the association chain of "gene expression - metabolic flux redistribution - physiological function improvement", and finally clarify the molecular - metabolic synergistic mechanism by which bacterial volatiles alleviate salt stress in cucumber, so as to provide a theoretical basis for the improvement of crop salt tolerance.

本研究以"细菌挥发性物-黄瓜-盐胁迫"互作系统为研究对象，依托转录组学（transcriptomics）与代谢组学（metabolomics）技术，确立核心目标如下：通过对比盐胁迫组、细菌挥发性物+盐胁迫组与对照组的黄瓜叶片和根系样本数据，筛选受细菌挥发性物调控的差异表达基因与特征代谢物；借助多组学关联分析构建"基因-代谢物"调控网络，阐明"基因表达-代谢流重分配-生理功能提升"的关联链条，最终明确细菌挥发性物缓解黄瓜盐胁迫的分子-代谢协同机制，为作物耐盐性改良提供理论依据。