ExtFig9C_TCID50_M-mut-WT-dX-24h_R1_LG389.HEIC

MDCK cells were infected at MOI 0.05 with the indicated viruses. Supernatants were collected at 1, 8, 24 and 48 hours post infection and viral titers were quantified by TCID50. Images show the TCID50 plates with scoring.

以感染复数（Multiplicity of Infection, MOI）0.05，将指定病毒接种至马-达二氏犬肾细胞（Madin-Darby canine kidney, MDCK）中。分别于感染后1、8、24及48小时收集细胞培养上清液，采用50%组织细胞感染量（50% Tissue Culture Infectious Dose, TCID₅₀）法对病毒滴度进行定量检测。本数据集附带的图像展示了带有计分判读结果的TCID₅₀检测板。