Pancreatic islet epigenomics reveals enhancer clusters that are enriched in Type 2 diabetes risk variants

We combined ChIP-seq of chromatin marks and key islet transcription factor with RNA-seq in human islets to map cis-regulatory networks in this primary tissue. The output of this project provides a reference map to dissect genetic variants that alter the susceptibility for Type 2 diabetes, and assist efforts to generate new beta-cells by transcriptional programming strategies

本研究在人类胰岛样本中，将针对染色质修饰标记与胰岛关键转录因子的染色质免疫共沉淀测序（ChIP-seq）与RNA测序（RNA-seq）相结合，以绘制该原代组织的顺式调控网络图谱。本项目产出的参考图谱可用于解析会改变2型糖尿病易感性的遗传变异，同时助力通过转录编程策略生成新型β细胞的相关研究。